Genetic modification in horticultural crops, particularly within the Cucurbitaceae family, is often hindered by complex tissue culture requirements and environmental pressures such as climate change. Traditional transformation techniques, like Agrobacterium-mediated gene transfer, frequently encounter barriers that limit their success in certain plant species. Genetic modification in horticultural crops, particularly within the Cucurbitaceae family, is often hindered by complex tissue culture requirements and environmental pressures such as climate change. Traditional transformation techniques, like Agrobacterium-mediated gene transfer, frequently encounter barriers that limit their success in certain plant species. Biotechnology Agriculture Phys.org – latest science and technology news stories